Journal: BMC Cancer

Article Title: Increased cytoplasmic and nuclear S100A6 expression is associated with improved prognosis in ovarian cancer

doi: 10.1186/s12885-026-15631-0

Figure Lengend Snippet: ( A ) Western blot of S100A6 in OVCA433 ovarian cancer cells, with an expected single band of 10 kDa. Full-length blots with an additional cell line (SKOV-3) are presented in Supplementary Fig. 2. Representative images of IHC staining for low ( B-D ) and high ( E-G ) cytoplasmic and nuclear expression of S100A6. 10X images with a 20X index box. Scale bar 100 μm

Article Snippet: Specificity of the S100A6 antibody (10245-1-AP, Proteintech) was initially determined via Western Blotting, to assess suitability for subsequent IHC based studies.

Techniques: Western Blot, Immunohistochemistry, Expressing

Journal: BMC Cancer

Article Title: Increased cytoplasmic and nuclear S100A6 expression is associated with improved prognosis in ovarian cancer

doi: 10.1186/s12885-026-15631-0

Figure Lengend Snippet: Kaplan-Meier analysis of ovarian cancer overall survival with low (black) and high (red) S100A6 expression in the cytoplasm ( A ) and nucleus ( B ). Significance was determined using the log-rank test

Article Snippet: Specificity of the S100A6 antibody (10245-1-AP, Proteintech) was initially determined via Western Blotting, to assess suitability for subsequent IHC based studies.

Techniques: Expressing

Journal: BMC Cancer

Article Title: Increased cytoplasmic and nuclear S100A6 expression is associated with improved prognosis in ovarian cancer

doi: 10.1186/s12885-026-15631-0

Figure Lengend Snippet: Kaplan-Meier analysis of ovarian cancer overall survival with low (black) and high (red) S100A6 expression in the cytoplasm ( A , C , E ) and nucleus ( B , D , F ) in high-grade serous carcinoma (HGSC) ( A - B ), mucinous carcinoma ( C - D ), and endometrioid carcinoma ( E - F ). Significance was determined using the log-rank test

Article Snippet: Specificity of the S100A6 antibody (10245-1-AP, Proteintech) was initially determined via Western Blotting, to assess suitability for subsequent IHC based studies.

Techniques: Expressing

Journal: BMC Cancer

Article Title: Increased cytoplasmic and nuclear S100A6 expression is associated with improved prognosis in ovarian cancer

doi: 10.1186/s12885-026-15631-0

Figure Lengend Snippet: Kaplan-Meier analysis of overall survival ( A ) and progression free survival ( B ) with low (black) and high (red) mRNA expression of S100A6 in ovarian cancer. Significance was determined using the log-rank test

Article Snippet: Specificity of the S100A6 antibody (10245-1-AP, Proteintech) was initially determined via Western Blotting, to assess suitability for subsequent IHC based studies.

Techniques: Expressing

Journal: Cell Communication and Signaling : CCS

Article Title: Physiological and pathological roles of ANXA11: a multifunctional regulator in neurodegeneration and other disorders

doi: 10.1186/s12964-025-02629-7

Figure Lengend Snippet: Structural Features of ANXA11. ( A ) Schematic diagrammatic interpretation of ANXA11 protein. ANXA11 contains an N-terminal low-complexity domain (LCD) and four C-terminal annexin repeat domains (ARD) forming Ca²⁺-binding motifs (visualized with IBS2.0). The amino acid residues 1–177 represent the prion-like domain predicted by PLAAC. The orange oval represents the structural domain of ANXA11 and calcyclin/S100A6 binding. The amino acid residues 39–74 indicate the regions involved in forming heteromeric amyloid filaments with TDP-43, as identified by cryo-EM studies. ( B ) Predicted 3D structure of ANXA11 showing C-terminal four ARDs (red) and the N-terminal LCD (purple), with Ca²⁺ binding sites. Predicted by AlphaFold and visualized using PyMOL Molecular Graphics System

Article Snippet: In proteostasis, S100A6 binding serves to dissolve and clear amyloid fibrils of mutant ANXA11, a protective function that is specifically compromised by ALS-associated mutations [ , ]. (2) The C-terminal core domain (encoded by exons 6–15) contains four evolutionarily conserved annexin repeat domains (ARD) that form canonical Ca2+-binding motifs, maintaining the characteristic Ca2+-dependent membrane association shared across the annexin superfamily [ – ].

Techniques: Binding Assay, Cryo-EM Sample Prep

Journal: Cell Communication and Signaling : CCS

Article Title: Physiological and pathological roles of ANXA11: a multifunctional regulator in neurodegeneration and other disorders

doi: 10.1186/s12964-025-02629-7

Figure Lengend Snippet: Multifunctional roles of ANXA11 in membrane-associated processes. ( A ) Membrane-Coupling and Membrane-associated Trafficking: In ER to Golgi apparatus transport, ANXA11 assembles with Sec31A and Ca²⁺-binding protein ALG-2 to form a Ca²⁺-dependent ternary complex at ERES membranes, maintaining the structural integrity of these membrane microdomains; ANXA11 maintains nuclear envelope stability through lamin B2 network organization, while mutations induce lamin B2 mislocalization and nuclear envelope disassembly; in cholangiocytes, ANXA11 mediates translocation of ANO1 chloride channels to the apical membrane, maintaining the biliary HCO3− umbrella; in pancreatic β-cells, ANXA11 localizes to insulin granules; in neutrophils, ANXA11 fragment selectively translocates to specific granule membranes in a Ca²⁺-dependent manner. ( B ) Phase Separation and Membraneless Organelles Trafficking: In neurons, ANXA11 tethers RNA granules to lysosomes through N-terminal LCD-mediated phase separation and C-terminal ARD membrane anchoring. This “hitchhiking” mechanism enables coupled protein-lipid phase transitions, impaired by ALS mutations. ( C ) Cell Cycle Regulation: ANXA11 dynamically relocalizes during mitosis to orchestrate cell division: initially responding to Ca²⁺ signals by associating with the nuclear envelope in complex with S100A6 during prophase, subsequently localizing to spindle poles in metaphase coinciding with S100A6 degradation, then migrating to the spindle midzone during anaphase, and finally integrating into the midbody structure during telophase to enable cytokinesis. ANXA11 is indispensable for proper midbody formation and successful cell division, as its depletion leads to cytokinesis failure

Article Snippet: In proteostasis, S100A6 binding serves to dissolve and clear amyloid fibrils of mutant ANXA11, a protective function that is specifically compromised by ALS-associated mutations [ , ]. (2) The C-terminal core domain (encoded by exons 6–15) contains four evolutionarily conserved annexin repeat domains (ARD) that form canonical Ca2+-binding motifs, maintaining the characteristic Ca2+-dependent membrane association shared across the annexin superfamily [ – ].

Techniques: Membrane, Binding Assay, Translocation Assay